Biofuel Testing Services

ECA offers a complete array of Biofuel, Biofuel Feedstock, and Biofuel Production By-Products testing services. Our years of experience in custom chemical analysis and our collective background in analytical method development allow us to offer complete characterization of Biofuel Feedstock. We can go beyond most other laboratories in the analysis of difficult samples. We can test Biodiesel per ASTM D6751 specifications or EN 14214-03 specifications. We test Biodiesel or Biodiesel Feedstock per any of the ASTM/EN methods or the AOCS methods. We have also developed methods for the analysis of chlorophyll, waxes, complete unsaponifiable profile, and complete phospholipid profile to help provide a further understanding of the Biofuel Feestock and Biofuel.

Please call Jim Polansky @ 858-535-9979 or email to jpolansky@ecalab.com for further information.

Biofuel Testing/ASTM/EN/AOCS Test procedures
ASTM Testing Procedures per ASTM D675

Name of Test

Method

Kinematic Viscosity (@40°C)

ASTM D445

Ash Content

ASTM D482

Sulfated Ash

ASTM D874

Moisture

ASTM D6305

Biodiesel Fuel Blend Verification

ASTM D7371

Cloud Point

ASTM D2500

Pour Point

ASTM D97

Sediment and Water

ASTM D2709

API Specific Gravity

ASTM D1298

Simulated Distillation

ASTM D2887

Elemental Scan/ICP-AES

ASTM D4951

Free and Total Glycerin

ASTM D6584

API Gravity

ASTM D1298/D4052

Flash Point

ASTM D93

Carbon Residue

ASTM D4530

Cetaine Number

ASTM D613

Derived Cetaine Number

ASTM D6890

Acid Number

ASTM D664

Phosphorus/ICP

ASTM D4951 mod.

Distillation 90% Recovered

ASTM D1160


Analytical Guidelines for Assessing Feedstock to Ensure Biodiesel Quality/
AOCS Ck 1-07

Name of Test

Method

Unsaponifiable Matter

AOCS Ca 6b-53/Ca 6a-40

Sampling

AOCS C 1-47

Insoluble Impurities

AOCS Ca 3a-46

Sediments by Centrifugation

AOCS Ca 3d-02

Soap in Oil

AOCS Cc 17-95

Polymerized Triglycerides by Gel Permeation HPLC

AOCS Cd 22-91

Water by Karl Fischer

AOCS Ca 2e-84

Modified Moisture and Volatiles

AOCS Ca 2f-93

Acid Value

AOCS Cd 3d-63

Sulfur in Oil/ICP-AES

AOCS Ca 17-01

Phospholipids in Vegetable Oil

AOCS Ca 19-86

Phosphorus in Oil/ICP-AES

AOCS Ca 20-99

Fat Stability/Peroxide Value

AOCS Cd 8b-90

p-Anisidine Value

AOCS Cd 18-90

Fatty Acid Composition

AOCS Ce 1-62

Calculated Iodine Value

AOCS Cd 1c-85

Free Fatty Acids

AOCS Ca 5a-40

Iodine Value

AOCS Cd 1d-92

Lead Content

AOCS Ca 18c-91

Saponification Value

AOCS Cd  3-25

Smoke Point

AOCS Cc 9a-48

 

Biodiesel Testing per EN 14214-03 Specifications

Name of Test

Method

Density @15.0°C

EN 12185

Kinematic Viscosoty @40.0°C

EN ISO 3104

Carbon Residue

EN 10370

Ester Content

EN 14103

Cloud Point

EN 23015

Sulfated Ash

EN ISO 3987

Methanol /Ethanol Content

EN 14110

Sulfur Content

EN ISO 20846/20884

Cetaine Number

EN ISO 5165

Water Content

EN ISO 12937

Total Contamination

EN 12662

Acid Value

EN 14104

Iodine value

EN 14111

Linolenic Acid Methyl Ester

EN 14103

Methanol

EN 14110

Free Glycerol/Total Glycerol Monoglycerides, Diglycerides, Triglycerides

EN 14105/14106

Alkali Metals

EN 14538

Phosphorus Content

EN 14107

 

Biodiesel By-Product Testing/Animal Feed Testing

Name of Test

Method

Amino Acid Analysis

AOAC 982.30

Ash

AOAC 942.05

Calcium

AOAC 985.01

Chloride

AOAC 943.01

Carbohydrate

Calculation

Fat (ether extract)

AOAC 920.39

Fiber (crude)

AOAC 978.10

Moisture

AOAC 934.01

Minerals

AOAC 985.01

Phosphorus

AOAC 985.01

Pepsin Digestibility

AOAC 971.09

TDN (Total Digestible Nutrient)

calculation

Calories

Calculation

Crude Protein

AOAC 984.13

 

Other Testing Procedures for Biofuel and Biofuel- Feedstock Testing

Name of  Test

Method

Protein Profile

SDS-PAGE

Wax Content of Oil

GC-FID

Phospholipid Profile

HPLC

Unsaponifiables Profile

GC-MS

Polyunsaturated Methyl Esters

GC-FID

CHNOS combustion elemental analysis

Combustion Elemental Analysis

Chlorophyll Content in Oils

AOCS Cc 13d-55(09)